Reporter
T7-GFP

Part:BBa_I719015

Designed by: Imperial 2007   Group: iGEM07_Imperial   (2007-10-24)

T7 GFPmut3b

This part was derived by the ligating parts BBa_I719005 and BBa_I13504. Has a minor design issue which does not affect functionality of part. This part has been characterized as BBa_I719005.

Usage and Biology

This construct acts as reporter system for the T7 promoter. This part has several advantages over its related part BBa_E7104, including a stronger RBS for enhanced protein expression, and a stronger double terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


[edit]
Categories
Parameters
None